Coding
gltB
Part:BBa_K367011:Design
Designed by: Fernando Govantes Group: iGEM10_UPO-Sevilla (2010-06-30)
gltB coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1404
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 826
Illegal AgeI site found at 3964
Illegal AgeI site found at 4093 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 348
Illegal SapI.rc site found at 1489
Illegal SapI.rc site found at 4363
Design Notes
No editing, except for modification of the stop codon to match the biobrick standard double stop codon
Source
Escherichia coli str. K-12 substr. MG1655, complete genome GenBank: U00096.2